International Commission on Trichinellosis (ICT) Recommendations for Quality Assurance in Digestion Testing Programs for Trichinella ICT Quality Assurance Committee (Appendix 1) Part 2 Essential quality assurance standards for Trichinella digestion assays

C. CRITICAL CONTROL POINTS AND MINIMUM STANDARDS 2 Figure 1: Example of a magnetic stirrer method for pooled sample digestion (steps labelled as 1-4 indicate the required sequential order for preparing the digest). 3 C1 Sample collection and preparation for testing 4 C2 Minimum requirements for equipment and consumables 5 C3 Performance of the digestion assay 6 C3.1 Blending of muscle samples 6 C3.2 Preparation of the digest fluid 6 C3.3 Digestion of chopped meat in the glass beaker 7 C3.4 Filtration of the digest fluid 7 C3.5 Sedimentation of the digest fluid in the separatory funnel 7 C3.6 Collection of the primary and secondary sediment 8 C3.7 Microscopic examination 8 C4 Verification of findings 9 C5 Documentation 9